Active Replication of Middle East Respiratory Syndrome Coronavirus and Aberrant Induction of Inflammatory Cytokines and Chemokines in Human Macrophages: Implications for Pathogenesis
Identifieur interne : 001997 ( Main/Exploration ); précédent : 001996; suivant : 001998Active Replication of Middle East Respiratory Syndrome Coronavirus and Aberrant Induction of Inflammatory Cytokines and Chemokines in Human Macrophages: Implications for Pathogenesis
Auteurs : Jie Zhou ; Hin Chu ; Cun Li ; Bosco Ho-Yin Wong ; Zhong-Shan Cheng ; Vincent Kwok-Man Poon ; Tianhao Sun ; Candy Choi-Yi Lau ; Kenneth Kak-Yuen Wong [République populaire de Chine] ; Jimmy Yu-Wai Chan [République populaire de Chine] ; Jasper Fuk-Woo Chan ; Kelvin Kai-Wang To ; Kwok-Hung Chan ; Bo-Jian Zheng ; Kwok-Yung YuenSource :
- The Journal of Infectious Diseases [ 0022-1899 ] ; 2013.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Cellules cultivées, Coronavirus (immunologie), Coronavirus (pathogénicité), Coronavirus (physiologie), Cytokines (biosynthèse), Cytokines (immunologie), Humains, Infections à coronavirus (immunologie), Infections à coronavirus (virologie), Macrophages (immunologie), Macrophages (virologie), Pneumopathie virale (immunologie), Pneumopathie virale (virologie), Poumon (virologie), Présentation d'antigène, Réplication virale (physiologie), Survie cellulaire (immunologie).
- MESH :
- biosynthèse : Cytokines.
- immunologie : Coronavirus, Cytokines, Infections à coronavirus, Macrophages, Pneumopathie virale, Survie cellulaire.
- pathogénicité : Coronavirus.
- physiologie : Coronavirus, Réplication virale.
- virologie : Infections à coronavirus, Macrophages, Pneumopathie virale, Poumon.
- Animaux, Cellules Vero, Cellules cultivées, Humains, Présentation d'antigène.
English descriptors
- KwdEn :
- Animals, Antigen Presentation, Cell Survival (immunology), Cells, Cultured, Chlorocebus aethiops, Coronavirus (immunology), Coronavirus (pathogenicity), Coronavirus (physiology), Coronavirus Infections (immunology), Coronavirus Infections (virology), Cytokines (biosynthesis), Cytokines (immunology), Humans, Lung (virology), Macrophages (immunology), Macrophages (virology), Pneumonia, Viral (immunology), Pneumonia, Viral (virology), Vero Cells, Virus Replication (physiology).
- MESH :
- chemical , biosynthesis : Cytokines.
- immunology : Cell Survival, Coronavirus, Coronavirus Infections, Cytokines, Macrophages, Pneumonia, Viral.
- pathogenicity : Coronavirus.
- physiology : Coronavirus, Virus Replication.
- virology : Coronavirus Infections, Lung, Macrophages, Pneumonia, Viral.
- Animals, Antigen Presentation, Cells, Cultured, Chlorocebus aethiops, Humans, Vero Cells.
Abstract
Middle East respiratory syndrome coronavirus (MERS-CoV) infection caused severe pneumonia and multiorgan dysfunction and had a higher crude fatality rate (around 50% vs 10%) than SARS coronavirus (SARS-CoV) infection. To understand the pathogenesis, we studied viral replication, cytokine/chemokine response, and antigen presentation in MERS-CoV–infected human monocyte–derived macrophages (MDMs) versus SARS-CoV–infected MDMs. Only MERS-CoV can replicate in MDMs. Both viruses were unable to significantly stimulate the expression of antiviral cytokines (interferon α [IFN-α] and IFN-β) but induced comparable levels of tumor necrosis factor α and interleukin 6. Notably, MERS-CoV induced significantly higher expression levels of interleukin 12, IFN-γ, and chemokines (IP-10/CXCL-10, MCP-1/CCL-2, MIP-1α/CCL-3, RANTES/CCL-5, and interleukin 8) than SARS-CoV. The expression of major histocompatibility complex class I and costimulatory molecules were significantly higher in MERS-CoV–infected MDMs than in SARS-CoV–infected cells. MERS-CoV replication was validated by immunostaining of infected MDMs and ex vivo lung tissue. We conclusively showed that MERS-CoV can establish a productive infection in human macrophages. The aberrant induction of inflammatory cytokines/chemokines could be important in the disease pathogenesis.
Url:
DOI: 10.1093/infdis/jit504
PubMed: 24065148
PubMed Central: 7107356
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 001172
- to stream Pmc, to step Curation: 001172
- to stream Pmc, to step Checkpoint: 000A11
- to stream PubMed, to step Corpus: 001123
- to stream PubMed, to step Curation: 001123
- to stream PubMed, to step Checkpoint: 001050
- to stream Ncbi, to step Merge: 002756
- to stream Ncbi, to step Curation: 002756
- to stream Ncbi, to step Checkpoint: 002756
- to stream Main, to step Merge: 001A07
- to stream Main, to step Curation: 001997
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Active Replication of Middle East Respiratory Syndrome Coronavirus and Aberrant Induction of Inflammatory Cytokines and Chemokines in Human Macrophages: Implications for Pathogenesis</title><author><name sortKey="Zhou, Jie" sort="Zhou, Jie" uniqKey="Zhou J" first="Jie" last="Zhou">Jie Zhou</name><affiliation><nlm:aff id="af1"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chu, Hin" sort="Chu, Hin" uniqKey="Chu H" first="Hin" last="Chu">Hin Chu</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Li, Cun" sort="Li, Cun" uniqKey="Li C" first="Cun" last="Li">Cun Li</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Wong, Bosco Ho Yin" sort="Wong, Bosco Ho Yin" uniqKey="Wong B" first="Bosco Ho-Yin" last="Wong">Bosco Ho-Yin Wong</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cheng, Zhong Shan" sort="Cheng, Zhong Shan" uniqKey="Cheng Z" first="Zhong-Shan" last="Cheng">Zhong-Shan Cheng</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Poon, Vincent Kwok Man" sort="Poon, Vincent Kwok Man" uniqKey="Poon V" first="Vincent Kwok-Man" last="Poon">Vincent Kwok-Man Poon</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Sun, Tianhao" sort="Sun, Tianhao" uniqKey="Sun T" first="Tianhao" last="Sun">Tianhao Sun</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Lau, Candy Choi Yi" sort="Lau, Candy Choi Yi" uniqKey="Lau C" first="Candy Choi-Yi" last="Lau">Candy Choi-Yi Lau</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Wong, Kenneth Kak Yuen" sort="Wong, Kenneth Kak Yuen" uniqKey="Wong K" first="Kenneth Kak-Yuen" last="Wong">Kenneth Kak-Yuen Wong</name><affiliation wicri:level="1"><nlm:aff id="af5"><addr-line>Department of Surgery</addr-line>,<institution>University of Hong Kong, Hong Kong Special Administrative Region</institution>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Jimmy Yu Wai" sort="Chan, Jimmy Yu Wai" uniqKey="Chan J" first="Jimmy Yu-Wai" last="Chan">Jimmy Yu-Wai Chan</name><affiliation wicri:level="1"><nlm:aff id="af5"><addr-line>Department of Surgery</addr-line>,<institution>University of Hong Kong, Hong Kong Special Administrative Region</institution>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name><affiliation><nlm:aff id="af1"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line></nlm:aff></affiliation></author><author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><affiliation><nlm:aff id="af1"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><affiliation><nlm:aff id="af1"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation><nlm:aff id="af1"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24065148</idno><idno type="pmc">7107356</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7107356</idno><idno type="RBID">PMC:7107356</idno><idno type="doi">10.1093/infdis/jit504</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">001172</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001172</idno><idno type="wicri:Area/Pmc/Curation">001172</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001172</idno><idno type="wicri:Area/Pmc/Checkpoint">000A11</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A11</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:24065148</idno><idno type="wicri:Area/PubMed/Corpus">001123</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001123</idno><idno type="wicri:Area/PubMed/Curation">001123</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001123</idno><idno type="wicri:Area/PubMed/Checkpoint">001050</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001050</idno><idno type="wicri:Area/Ncbi/Merge">002756</idno><idno type="wicri:Area/Ncbi/Curation">002756</idno><idno type="wicri:Area/Ncbi/Checkpoint">002756</idno><idno type="wicri:doubleKey">0022-1899:2013:Zhou J:active:replication:of</idno><idno type="wicri:Area/Main/Merge">001A07</idno><idno type="wicri:Area/Main/Curation">001997</idno><idno type="wicri:Area/Main/Exploration">001997</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Active Replication of Middle East Respiratory Syndrome Coronavirus and Aberrant Induction of Inflammatory Cytokines and Chemokines in Human Macrophages: Implications for Pathogenesis</title><author><name sortKey="Zhou, Jie" sort="Zhou, Jie" uniqKey="Zhou J" first="Jie" last="Zhou">Jie Zhou</name><affiliation><nlm:aff id="af1"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chu, Hin" sort="Chu, Hin" uniqKey="Chu H" first="Hin" last="Chu">Hin Chu</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Li, Cun" sort="Li, Cun" uniqKey="Li C" first="Cun" last="Li">Cun Li</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Wong, Bosco Ho Yin" sort="Wong, Bosco Ho Yin" uniqKey="Wong B" first="Bosco Ho-Yin" last="Wong">Bosco Ho-Yin Wong</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Cheng, Zhong Shan" sort="Cheng, Zhong Shan" uniqKey="Cheng Z" first="Zhong-Shan" last="Cheng">Zhong-Shan Cheng</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Poon, Vincent Kwok Man" sort="Poon, Vincent Kwok Man" uniqKey="Poon V" first="Vincent Kwok-Man" last="Poon">Vincent Kwok-Man Poon</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Sun, Tianhao" sort="Sun, Tianhao" uniqKey="Sun T" first="Tianhao" last="Sun">Tianhao Sun</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Lau, Candy Choi Yi" sort="Lau, Candy Choi Yi" uniqKey="Lau C" first="Candy Choi-Yi" last="Lau">Candy Choi-Yi Lau</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Wong, Kenneth Kak Yuen" sort="Wong, Kenneth Kak Yuen" uniqKey="Wong K" first="Kenneth Kak-Yuen" last="Wong">Kenneth Kak-Yuen Wong</name><affiliation wicri:level="1"><nlm:aff id="af5"><addr-line>Department of Surgery</addr-line>,<institution>University of Hong Kong, Hong Kong Special Administrative Region</institution>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Jimmy Yu Wai" sort="Chan, Jimmy Yu Wai" uniqKey="Chan J" first="Jimmy Yu-Wai" last="Chan">Jimmy Yu-Wai Chan</name><affiliation wicri:level="1"><nlm:aff id="af5"><addr-line>Department of Surgery</addr-line>,<institution>University of Hong Kong, Hong Kong Special Administrative Region</institution>,<country>China</country></nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name><affiliation><nlm:aff id="af1"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line></nlm:aff></affiliation></author><author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><affiliation><nlm:aff id="af1"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line></nlm:aff></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><affiliation><nlm:aff id="af1"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line></nlm:aff></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation><nlm:aff id="af1"><addr-line>State Key Laboratory of Emerging Infectious Diseases</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af2"><addr-line>Department of Microbiology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af3"><addr-line>Research Centre of Infection and Immunology</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="af4"><addr-line>Carol Yu Centre for Infection</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">The Journal of Infectious Diseases</title><idno type="ISSN">0022-1899</idno><idno type="eISSN">1537-6613</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antigen Presentation</term><term>Cell Survival (immunology)</term><term>Cells, Cultured</term><term>Chlorocebus aethiops</term><term>Coronavirus (immunology)</term><term>Coronavirus (pathogenicity)</term><term>Coronavirus (physiology)</term><term>Coronavirus Infections (immunology)</term><term>Coronavirus Infections (virology)</term><term>Cytokines (biosynthesis)</term><term>Cytokines (immunology)</term><term>Humans</term><term>Lung (virology)</term><term>Macrophages (immunology)</term><term>Macrophages (virology)</term><term>Pneumonia, Viral (immunology)</term><term>Pneumonia, Viral (virology)</term><term>Vero Cells</term><term>Virus Replication (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Cellules cultivées</term><term>Coronavirus (immunologie)</term><term>Coronavirus (pathogénicité)</term><term>Coronavirus (physiologie)</term><term>Cytokines (biosynthèse)</term><term>Cytokines (immunologie)</term><term>Humains</term><term>Infections à coronavirus (immunologie)</term><term>Infections à coronavirus (virologie)</term><term>Macrophages (immunologie)</term><term>Macrophages (virologie)</term><term>Pneumopathie virale (immunologie)</term><term>Pneumopathie virale (virologie)</term><term>Poumon (virologie)</term><term>Présentation d'antigène</term><term>Réplication virale (physiologie)</term><term>Survie cellulaire (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus</term><term>Cytokines</term><term>Infections à coronavirus</term><term>Macrophages</term><term>Pneumopathie virale</term><term>Survie cellulaire</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Cell Survival</term><term>Coronavirus</term><term>Coronavirus Infections</term><term>Cytokines</term><term>Macrophages</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Coronavirus</term><term>Réplication virale</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Coronavirus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term><term>Macrophages</term><term>Pneumopathie virale</term><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term><term>Lung</term><term>Macrophages</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antigen Presentation</term><term>Cells, Cultured</term><term>Chlorocebus aethiops</term><term>Humans</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Cellules cultivées</term><term>Humains</term><term>Présentation d'antigène</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p>Middle East respiratory syndrome coronavirus (MERS-CoV) infection caused severe pneumonia and multiorgan dysfunction and had a higher crude fatality rate (around 50% vs 10%) than SARS coronavirus (SARS-CoV) infection. To understand the pathogenesis, we studied viral replication, cytokine/chemokine response, and antigen presentation in MERS-CoV–infected human monocyte–derived macrophages (MDMs) versus SARS-CoV–infected MDMs. Only MERS-CoV can replicate in MDMs. Both viruses were unable to significantly stimulate the expression of antiviral cytokines (interferon α [IFN-α] and IFN-β) but induced comparable levels of tumor necrosis factor α and interleukin 6. Notably, MERS-CoV induced significantly higher expression levels of interleukin 12, IFN-γ, and chemokines (IP-10/CXCL-10, MCP-1/CCL-2, MIP-1α/CCL-3, RANTES/CCL-5, and interleukin 8) than SARS-CoV. The expression of major histocompatibility complex class I and costimulatory molecules were significantly higher in MERS-CoV–infected MDMs than in SARS-CoV–infected cells. MERS-CoV replication was validated by immunostaining of infected MDMs and ex vivo lung tissue. We conclusively showed that MERS-CoV can establish a productive infection in human macrophages. The aberrant induction of inflammatory cytokines/chemokines could be important in the disease pathogenesis.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author><author><name sortKey="Li, Ks" uniqKey="Li K">KS Li</name></author><author><name sortKey="To, Kk" uniqKey="To K">KK To</name></author><author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name></author><author><name sortKey="Chen, H" uniqKey="Chen H">H Chen</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name></author><author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name></author><author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Fouchier, Ra" uniqKey="Fouchier R">RA Fouchier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author><author><name sortKey="Lau, S" uniqKey="Lau S">S Lau</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author><author><name sortKey="To, Kk" uniqKey="To K">KK To</name></author><author><name sortKey="Tse, H" uniqKey="Tse H">H Tse</name></author><author><name sortKey="Jin, Dy" uniqKey="Jin D">DY Jin</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Wang, M" uniqKey="Wang M">M Wang</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Birch, Cj" uniqKey="Birch C">CJ Birch</name></author><author><name sortKey="Clothier, Hj" uniqKey="Clothier H">HJ Clothier</name></author><author><name sortKey="Seccull, A" uniqKey="Seccull A">A Seccull</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="El Sahly, Hm" uniqKey="El Sahly H">HM El-Sahly</name></author><author><name sortKey="Atmar, Rl" uniqKey="Atmar R">RL Atmar</name></author><author><name sortKey="Glezen, Wp" uniqKey="Glezen W">WP Glezen</name></author><author><name sortKey="Greenberg, Sb" uniqKey="Greenberg S">SB Greenberg</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name></author><author><name sortKey="Lai, St" uniqKey="Lai S">ST Lai</name></author><author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author><author><name sortKey="Osterhaus, Ad" uniqKey="Osterhaus A">AD Osterhaus</name></author><author><name sortKey="Stohr, K" uniqKey="Stohr K">K Stohr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, Js" uniqKey="Peiris J">JS Peiris</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y Guan</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheng, Vc" uniqKey="Cheng V">VC Cheng</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Hoek, L" uniqKey="Van Der Hoek L">L van der Hoek</name></author><author><name sortKey="Pyrc, K" uniqKey="Pyrc K">K Pyrc</name></author><author><name sortKey="Jebbink, Mf" uniqKey="Jebbink M">MF Jebbink</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Woo, Pc" uniqKey="Woo P">PC Woo</name></author><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Chu, Cm" uniqKey="Chu C">CM Chu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Assiri, A" uniqKey="Assiri A">A Assiri</name></author><author><name sortKey="Mcgeer, A" uniqKey="Mcgeer A">A McGeer</name></author><author><name sortKey="Perl, Tm" uniqKey="Perl T">TM Perl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reusken, Cb" uniqKey="Reusken C">CB Reusken</name></author><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name></author><author><name sortKey="Muller, Ma" uniqKey="Muller M">MA Muller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Annan, A" uniqKey="Annan A">A Annan</name></author><author><name sortKey="Baldwin, Hj" uniqKey="Baldwin H">HJ Baldwin</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lau, Sk" uniqKey="Lau S">SK Lau</name></author><author><name sortKey="Li, Ks" uniqKey="Li K">KS Li</name></author><author><name sortKey="Tsang, Ak" uniqKey="Tsang A">AK Tsang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author><author><name sortKey="Tse, H" uniqKey="Tse H">H Tse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guery, B" uniqKey="Guery B">B Guery</name></author><author><name sortKey="Poissy, J" uniqKey="Poissy J">J Poissy</name></author><author><name sortKey="El Mansouf, L" uniqKey="El Mansouf L">L el Mansouf</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Memish, Za" uniqKey="Memish Z">ZA Memish</name></author><author><name sortKey="Zumla, Ai" uniqKey="Zumla A">AI Zumla</name></author><author><name sortKey="Al Hakeem, Rf" uniqKey="Al Hakeem R">RF Al-Hakeem</name></author><author><name sortKey="Al Rabeeah, Aa" uniqKey="Al Rabeeah A">AA Al-Rabeeah</name></author><author><name sortKey="Stephens, Gm" uniqKey="Stephens G">GM Stephens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gu, J" uniqKey="Gu J">J Gu</name></author><author><name sortKey="Gong, E" uniqKey="Gong E">E Gong</name></author><author><name sortKey="Zhang, B" uniqKey="Zhang B">B Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nicholls, Jm" uniqKey="Nicholls J">JM Nicholls</name></author><author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name></author><author><name sortKey="Lee, Kc" uniqKey="Lee K">KC Lee</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Murray, Pj" uniqKey="Murray P">PJ Murray</name></author><author><name sortKey="Wynn, Ta" uniqKey="Wynn T">TA Wynn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Rw" uniqKey="Chan R">RW Chan</name></author><author><name sortKey="Chan, Mc" uniqKey="Chan M">MC Chan</name></author><author><name sortKey="Agnihothram, S" uniqKey="Agnihothram S">S Agnihothram</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kindler, E" uniqKey="Kindler E">E Kindler</name></author><author><name sortKey="Jonsdottir, Hr" uniqKey="Jonsdottir H">HR Jonsdottir</name></author><author><name sortKey="Muth, D" uniqKey="Muth D">D Muth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zielecki, F" uniqKey="Zielecki F">F Zielecki</name></author><author><name sortKey="Weber, M" uniqKey="Weber M">M Weber</name></author><author><name sortKey="Eickmann, M" uniqKey="Eickmann M">M Eickmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Jf" uniqKey="Chan J">JF Chan</name></author><author><name sortKey="Chan, Kh" uniqKey="Chan K">KH Chan</name></author><author><name sortKey="Choi, Gk" uniqKey="Choi G">GK Choi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chu, H" uniqKey="Chu H">H Chu</name></author><author><name sortKey="Wang, Jj" uniqKey="Wang J">JJ Wang</name></author><author><name sortKey="Qi, M" uniqKey="Qi M">M Qi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chu, H" uniqKey="Chu H">H Chu</name></author><author><name sortKey="Wang, Jj" uniqKey="Wang J">JJ Wang</name></author><author><name sortKey="Qi, M" uniqKey="Qi M">M Qi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, J" uniqKey="Zhou J">J Zhou</name></author><author><name sortKey="Tan, T" uniqKey="Tan T">T Tan</name></author><author><name sortKey="Tian, Y" uniqKey="Tian Y">Y Tian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, Kj" uniqKey="Huang K">KJ Huang</name></author><author><name sortKey="Su, Ij" uniqKey="Su I">IJ Su</name></author><author><name sortKey="Theron, M" uniqKey="Theron M">M Theron</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, Y" uniqKey="Jiang Y">Y Jiang</name></author><author><name sortKey="Xu, J" uniqKey="Xu J">J Xu</name></author><author><name sortKey="Zhou, C" uniqKey="Zhou C">C Zhou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cheung, Cy" uniqKey="Cheung C">CY Cheung</name></author><author><name sortKey="Poon, Ll" uniqKey="Poon L">LL Poon</name></author><author><name sortKey="Ng, Ih" uniqKey="Ng I">IH Ng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Seilmaier, M" uniqKey="Seilmaier M">M Seilmaier</name></author><author><name sortKey="Corman, Vm" uniqKey="Corman V">VM Corman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Raj, Vs" uniqKey="Raj V">VS Raj</name></author><author><name sortKey="Mou, H" uniqKey="Mou H">H Mou</name></author><author><name sortKey="Smits, Sl" uniqKey="Smits S">SL Smits</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Munster, Vj" uniqKey="Munster V">VJ Munster</name></author><author><name sortKey="De Wit, E" uniqKey="De Wit E">E de Wit</name></author><author><name sortKey="Feldmann, H" uniqKey="Feldmann H">H Feldmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Broxmeyer, He" uniqKey="Broxmeyer H">HE Broxmeyer</name></author><author><name sortKey="Sherry, B" uniqKey="Sherry B">B Sherry</name></author><author><name sortKey="Cooper, S" uniqKey="Cooper S">S Cooper</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tseng, Ct" uniqKey="Tseng C">CT Tseng</name></author><author><name sortKey="Perrone, La" uniqKey="Perrone L">LA Perrone</name></author><author><name sortKey="Zhu, H" uniqKey="Zhu H">H Zhu</name></author><author><name sortKey="Makino, S" uniqKey="Makino S">S Makino</name></author><author><name sortKey="Peters, Cj" uniqKey="Peters C">CJ Peters</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Orenstein, Jm" uniqKey="Orenstein J">JM Orenstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Jong, Md" uniqKey="De Jong M">MD de Jong</name></author><author><name sortKey="Simmons, Cp" uniqKey="Simmons C">CP Simmons</name></author><author><name sortKey="Thanh, Tt" uniqKey="Thanh T">TT Thanh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Law, Hk" uniqKey="Law H">HK Law</name></author><author><name sortKey="Cheung, Cy" uniqKey="Cheung C">CY Cheung</name></author><author><name sortKey="Ng, Hy" uniqKey="Ng H">HY Ng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ziegler, T" uniqKey="Ziegler T">T Ziegler</name></author><author><name sortKey="Matikainen, S" uniqKey="Matikainen S">S Matikainen</name></author><author><name sortKey="Ronkko, E" uniqKey="Ronkko E">E Ronkko</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kopecky Bromberg, Sa" uniqKey="Kopecky Bromberg S">SA Kopecky-Bromberg</name></author><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L Martinez-Sobrido</name></author><author><name sortKey="Frieman, M" uniqKey="Frieman M">M Frieman</name></author><author><name sortKey="Baric, Ra" uniqKey="Baric R">RA Baric</name></author><author><name sortKey="Palese, P" uniqKey="Palese P">P Palese</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name></author><author><name sortKey="Dandekar, Aa" uniqKey="Dandekar A">AA Dandekar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Josset, L" uniqKey="Josset L">L Josset</name></author><author><name sortKey="Menachery, Vd" uniqKey="Menachery V">VD Menachery</name></author><author><name sortKey="Gralinski, Le" uniqKey="Gralinski L">LE Gralinski</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><name sortKey="Cheng, Zhong Shan" sort="Cheng, Zhong Shan" uniqKey="Cheng Z" first="Zhong-Shan" last="Cheng">Zhong-Shan Cheng</name><name sortKey="Chu, Hin" sort="Chu, Hin" uniqKey="Chu H" first="Hin" last="Chu">Hin Chu</name><name sortKey="Lau, Candy Choi Yi" sort="Lau, Candy Choi Yi" uniqKey="Lau C" first="Candy Choi-Yi" last="Lau">Candy Choi-Yi Lau</name><name sortKey="Li, Cun" sort="Li, Cun" uniqKey="Li C" first="Cun" last="Li">Cun Li</name><name sortKey="Poon, Vincent Kwok Man" sort="Poon, Vincent Kwok Man" uniqKey="Poon V" first="Vincent Kwok-Man" last="Poon">Vincent Kwok-Man Poon</name><name sortKey="Sun, Tianhao" sort="Sun, Tianhao" uniqKey="Sun T" first="Tianhao" last="Sun">Tianhao Sun</name><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><name sortKey="Wong, Bosco Ho Yin" sort="Wong, Bosco Ho Yin" uniqKey="Wong B" first="Bosco Ho-Yin" last="Wong">Bosco Ho-Yin Wong</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><name sortKey="Zhou, Jie" sort="Zhou, Jie" uniqKey="Zhou J" first="Jie" last="Zhou">Jie Zhou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wong, Kenneth Kak Yuen" sort="Wong, Kenneth Kak Yuen" uniqKey="Wong K" first="Kenneth Kak-Yuen" last="Wong">Kenneth Kak-Yuen Wong</name></noRegion><name sortKey="Chan, Jimmy Yu Wai" sort="Chan, Jimmy Yu Wai" uniqKey="Chan J" first="Jimmy Yu-Wai" last="Chan">Jimmy Yu-Wai Chan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001997 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001997 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:7107356
|texte= Active Replication of Middle East Respiratory Syndrome Coronavirus and Aberrant Induction of Inflammatory Cytokines and Chemokines in Human Macrophages: Implications for Pathogenesis
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:24065148" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |